Document 3193
 DOCN  M94A3193
 TI    SIVmac chimeric viruses having HIV-1 env useful for HIV-1 vaccine
       evaluation using macaque monkeys.
 DT    9412
 AU    Kuwata T; Igarashi T; Okada M; Ido E; Ami Y; Komatsu T; Jin M; Adachi A;
       Hayami M; Institute for Virus Research, Kyoto University, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):131 (abstract no. PA0144). Unique
       Identifier : AIDSLINE ICA10/94369382
 AB    OBJECTIVE: By constructing SIVmac chimeric viruses having HIV-1 env more
       efficient in macaque infection, we attempts to establish an experimental
       infection system using macaque monkeys instead of chimpanzees. METHODS:
       The chimeric viruses having LTRs, gag, pol, vif and vpx of SIVmac and
       tat, rev, vpu and env of HIV-1 and additionally vpr and/or nef of either
       HIV-1 or SIVmac were constructed. Growth of chimeras was examined in
       human and macaque PBMC, and one of them was inoculated to macaques.
       RESULTS: Growth potential of each chimera was different depending on
       whether vpr and nef were from HIV-1, SIVmac or defective. When NM-3rN
       carrying HIV-1 vpr and SIVmac nef was inoculated to two cynomolgus and
       two rhesus monkeys, viruses were reisolated from all monkeys at 2
       through 14 weeks post inoculation constantly. DISCUSSION AND
       CONCLUSIONS: The results indicate the infection with NM-3rN is more
       efficient than that with previously constructed chimeric viruses, and
       suggest usefulness of NM-3rN as challenging virus for the HIV-1 vaccine
       evaluation in macaque monkeys. Now, macaques immunized with
       live-attenuated vaccine candidates were challenged with NM-3rN.
 DE    Animal  AIDS Vaccines/*PHARMACOLOGY  Chimera  Chimpansee troglodytes
       Comparative Study  Drug Evaluation  *Genes, env  *Genes, Viral
       HIV-1/*GENETICS/IMMUNOLOGY  Lymphocytes/MICROBIOLOGY  Macaca  Repetitive
       Sequences, Nucleic Acid  SIV/GROWTH & DEVELOPMENT/*GENETICS  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).